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pUC19_HDRT_TRAC_CD19-CAR
(Plasmid #183473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TRAC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Entrez Gene
    TRAC (a.k.a. IMD7, TCRA, TRA, TRCA)

Gene/Insert 2

  • Gene/Insert name
    CD19-specific CAR transgene insert
  • Species
    H. sapiens (human), B. taurus (bovine), Synthetic
  • Insert Size (bp)
    2015

Gene/Insert 3

  • Gene/Insert name
    TRAC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    399
  • Entrez Gene
    TRAC (a.k.a. IMD7, TCRA, TRA, TRCA)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_HDRT_TRAC_CD19-CAR was a gift from Dimitrios Wagner (Addgene plasmid # 183473 ; http://n2t.net/addgene:183473 ; RRID:Addgene_183473)

  • For your References section:

    Pharmacological interventions enhance virus-free generation of TRAC-replaced CAR T cells. Kath J, Du W, Pruene A, Braun T, Thommandru B, Turk R, Sturgeon ML, Kurgan GL, Amini L, Stein M, Zittel T, Martini S, Ostendorf L, Wilhelm A, Akyuz L, Rehm A, Hopken UE, Pruss A, Kunkele A, Jacobi AM, Volk HD, Schmueck-Henneresse M, Stripecke R, Reinke P, Wagner DL. Mol Ther Methods Clin Dev. 2022 Apr 12;25:311-330. doi: 10.1016/j.omtm.2022.03.018. eCollection 2022 Jun 9. 10.1016/j.omtm.2022.03.018 PubMed 35573047
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