pAAV-CBh-IgKL-mNG
(Plasmid
#183467)
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PurposeTo produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-Cbh-*-WPRE-SV40pA
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Backbone manufacturerGunma University, Hirai lab
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIgKL-mNeonGreen
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SpeciesSynthetic
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Tag
/ Fusion Protein
- IgKL secretory signal (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit for https://www.biorxiv.org/content/10.1101/2022.04.08.487498v1 bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CBh-IgKL-mNG was a gift from Hajime Hirase (Addgene plasmid # 183467 ; http://n2t.net/addgene:183467 ; RRID:Addgene_183467) -
For your References section:
Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. Wang X, Delle C, Asiminas A, Akther S, Vittani M, Brogger P, Kusk P, Vo CT, Radovanovic T, Konno A, Hirai H, Fukuda M, Weikop P, Goldman SA, Nedergaard M, Hirase H. Cell Rep Methods. 2022 Sep 21;2(10):100302. doi: 10.1016/j.crmeth.2022.100302. eCollection 2022 Oct 24. 10.1016/j.crmeth.2022.100302 PubMed 36313804