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Addgene

pAAV-CBh-IgKL-mNG
(Plasmid #183467)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183467 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-Cbh-*-WPRE-SV40pA
  • Backbone manufacturer
    Gunma University, Hirai lab
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgKL-mNeonGreen
  • Species
    Synthetic
  • Tag / Fusion Protein
    • IgKL secretory signal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CBh-IgKL-mNG was a gift from Hajime Hirase (Addgene plasmid # 183467 ; http://n2t.net/addgene:183467 ; RRID:Addgene_183467)
  • For your References section:

    Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. Wang X, Delle C, Asiminas A, Akther S, Vittani M, Brogger P, Kusk P, Vo CT, Radovanovic T, Konno A, Hirai H, Fukuda M, Weikop P, Goldman SA, Nedergaard M, Hirase H. Cell Rep Methods. 2022 Sep 21;2(10):100302. doi: 10.1016/j.crmeth.2022.100302. eCollection 2022 Oct 24. 10.1016/j.crmeth.2022.100302 PubMed 36313804