pAAV-P3-Alb-mScarlet
(Plasmid #183461)

• Purpose: To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein
• Depositing Lab: Hajime Hirase
• Publication: Wang et al Cell Rep Methods. 2022 Sep 21;2(10):100302. doi: 10.1016/j.crmeth.2022.100302. eCollection 2022 Oct 24.

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Backbone

• Vector backbone: pAAV-P3
• Backbone manufacturer: VVF (p438) https://vvf.ethz.ch/
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alb
• Species: M. musculus (mouse)
• Entrez Gene: Alb (a.k.a. Alb-1, Alb1, BCL002)
• Tag / Fusion Protein:
  • mScarlet (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit for https://www.biorxiv.org/content/10.1101/2022.04.08.487498v1 bioRxiv preprint.