pcDNA3_2FLAG-AKAP79ΔCN
(Plasmid #183451)

• Purpose: Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)
• Depositing Lab: Matthew Gold
• Publication: Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi: 10.1038/s41467-017-01715-w.

Backbone

• Vector backbone: pcDNA3.1D V5-His-TOPO
• Backbone manufacturer: ThermoFisher Scientific
• Backbone size w/o insert (bp): 5514
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AKAP5
• Alt name: AKAP79
• Species: H. sapiens (human)
• Insert Size (bp): 1263
• Mutation: aa337-343 deleted
• Entrez Gene: AKAP5 (a.k.a. AKAP75, AKAP79, H21)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7F
• 3′ sequencing primer: bGHR

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The coding sequence was originally cloned by John Scott: https://www.ncbi.nlm.nih.gov/nuccore/178323

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry