pHBT-Mb(GDx-Clo_L10)
(Plasmid #183406)

• Purpose: Expresses Monobody GDx-Clo_L10 in E. coli
• Depositing Lab: Shohei Koide
• Publication: Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi: 10.1038/s41467-020-19820-8.

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5310
• Total vector size (bp): 5612
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21(DE3) for protein expression. Grow at 37°C until OD600 is 0.8 and add final 0.2 mM IPTG. After IPTG induction, reduce the temperature to 18°C and grow overnight.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
• Species: Synthetic
• Insert Size (bp): 372
• Promoter: T7
• Tag / Fusion Protein:
  • His tag-Avi tag-TEV cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7promoter
• 3′ sequencing primer: T7terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry