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Addgene

pHBT-Mb(GDx-Clo_L10)
(Plasmid #183406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5310
  • Total vector size (bp) 5612
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for protein expression. Grow at 37°C until OD600 is 0.8 and add final 0.2 mM IPTG. After IPTG induction, reduce the temperature to 18°C and grow overnight.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
  • Species
    Synthetic
  • Insert Size (bp)
    372
  • Promoter T7
  • Tag / Fusion Protein
    • His tag-Avi tag-TEV cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7promoter
  • 3′ sequencing primer T7terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBT-Mb(GDx-Clo_L10) was a gift from Shohei Koide (Addgene plasmid # 183406 ; http://n2t.net/addgene:183406 ; RRID:Addgene_183406)

  • For your References section:

    The structural basis of promiscuity in small multidrug resistance transporters. Kermani AA, Macdonald CB, Burata OE, Ben Koff B, Koide A, Denbaum E, Koide S, Stockbridge RB. Nat Commun. 2020 Nov 27;11(1):6064. doi: 10.1038/s41467-020-19820-8. 10.1038/s41467-020-19820-8 PubMed 33247110
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