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Addgene

NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1
(Plasmid #183404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1 was a gift from Alice Ting (Addgene plasmid # 183404 ; http://n2t.net/addgene:183404 ; RRID:Addgene_183404)

  • For your References section:

    A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells. Erdenee E, Ting AY. ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 10.1021/acssynbio.1c00597. Epub 2022 Mar 7. 10.1021/acssynbio.1c00597 PubMed 35254056
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