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Addgene

NLS-LgBiT-FRB
(Plasmid #183403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LgBiT-FRB
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-LgBiT-FRB was a gift from Alice Ting (Addgene plasmid # 183403 ; http://n2t.net/addgene:183403 ; RRID:Addgene_183403)

  • For your References section:

    A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells. Erdenee E, Ting AY. ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 10.1021/acssynbio.1c00597. Epub 2022 Mar 7. 10.1021/acssynbio.1c00597 PubMed 35254056
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