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pMSCV-IRES-GFP-human-caspase-1/4b
(Plasmid #183394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-EGFP
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARD domain of human caspase-1 fused to human caspase-4
  • Alt name
    hCARD1 + 4/mEnz4
  • Alt name
    human caspase-1/4b
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1500
  • Entrez Gene
    CASP1 (a.k.a. ICE, IL1BC, P45)
  • Promoter MESV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer MSCV-fwd
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-GFP-human-caspase-1/4b was a gift from Jonathan Kagan (Addgene plasmid # 183394 ; http://n2t.net/addgene:183394 ; RRID:Addgene_183394)

  • For your References section:

    Evolution-inspired redesign of the LPS receptor caspase-4 into an interleukin-1beta converting enzyme. Devant P, Cao A, Kagan JC. Sci Immunol. 2021 Aug 10;6(62). doi: 10.1126/sciimmunol.abh3567. 10.1126/sciimmunol.abh3567 PubMed 34734155
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