pET28_mouse-pro-IL18
(Plasmid
#183389)
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PurposeOverexpression of catalytic domain of inflammatory caspase in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepro-IL18
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Alt nameInterleukin 18 precursor
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SpeciesM. musculus (mouse)
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Insert Size (bp)950
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Entrez GeneIl18 (a.k.a. Igif, Il-18)
- Promoter T7
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Tag
/ Fusion Protein
- His-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7-fwd
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28_mouse-pro-IL18 was a gift from Jonathan Kagan (Addgene plasmid # 183389 ; http://n2t.net/addgene:183389 ; RRID:Addgene_183389) -
For your References section:
Evolution-inspired redesign of the LPS receptor caspase-4 into an interleukin-1beta converting enzyme. Devant P, Cao A, Kagan JC. Sci Immunol. 2021 Aug 10;6(62). doi: 10.1126/sciimmunol.abh3567. 10.1126/sciimmunol.abh3567 PubMed 34734155