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pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
(Plasmid #183387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    redesigned Caspase-4 (AA94-377)
  • Alt name
    Caspase-4 quadruple mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    ANR261ENP, D315H, M318K, T352K
  • Entrez Gene
    CASP4 (a.k.a. ICE(rel)II, ICEREL-II, ICH-2, Mih1, Mih1/TX, TX)
  • Promoter T7
  • Tag / Fusion Protein
    • His-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7-fwd
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain was a gift from Jonathan Kagan (Addgene plasmid # 183387 ; http://n2t.net/addgene:183387 ; RRID:Addgene_183387)

  • For your References section:

    Evolution-inspired redesign of the LPS receptor caspase-4 into an interleukin-1beta converting enzyme. Devant P, Cao A, Kagan JC. Sci Immunol. 2021 Aug 10;6(62). doi: 10.1126/sciimmunol.abh3567. 10.1126/sciimmunol.abh3567 PubMed 34734155
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