pET28_human-Caspase-4_enzymatic-domain
(Plasmid #183381)

• Purpose: Overexpression of catalytic domain of inflammatory caspase in bacteria
• Depositing Lab: Jonathan Kagan
• Publication: Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.1126/sciimmunol.abh3567.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase-4 (AA94-377)
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Entrez Gene: CASP4 (a.k.a. ICE(rel)II, ICEREL-II, ICH-2, Mih1, Mih1/TX, TX)
• Promoter: T7
• Tag / Fusion Protein:
  • His-TEV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7-fwd
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry