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Addgene

CMV-H2B-sfGFP-T2A-iCre
(Plasmid #183351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183351 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H2B-sfGFP-T2A-iCre
  • Species
    H. sapiens (human), Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CMV promoter is from Invitrogen. HIST1H2BB is from Addgene plasmid #20972. sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function (similar to Addgene plasmids #113837 and #113849).

The vector backbone is a modified Invitrogen's pcDNA3. The NeoR cassette was partially truncated during cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-H2B-sfGFP-T2A-iCre was a gift from Mario Capecchi (Addgene plasmid # 183351 ; http://n2t.net/addgene:183351 ; RRID:Addgene_183351)
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