pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)
(Plasmid
#183254)
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PurposeExpression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTF-pcDNA3.1
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTissue factor
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SpeciesH. sapiens (human)
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Entrez GeneF3 (a.k.a. CD142, TF, TFA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBbiRP Hu Full Length CD142 (Tissue Factor; F3) was a gift from Alexander McLellan (Addgene plasmid # 183254 ; http://n2t.net/addgene:183254 ; RRID:Addgene_183254) -
For your References section:
Procoagulant and immunogenic properties of melanoma exosomes, microvesicles and apoptotic vesicles. Muhsin-Sharafaldine MR, Saunderson SC, Dunn AC, Faed JM, Kleffmann T, McLellan AD. Oncotarget. 2016 Aug 30;7(35):56279-56294. doi: 10.18632/oncotarget.10783. 10.18632/oncotarget.10783 PubMed 27462921