pcDNA3.1(-) HuTF-Fc-His tag
(Plasmid
#183253)
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PurposeSoluble expression of the extracellular domains of human tissue factor (TF)-Fc
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTissue factor
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SpeciesH. sapiens (human)
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Entrez GeneF3 (a.k.a. CD142, TF, TFA)
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Tag
/ Fusion Protein
- 8xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(-) HuTF-Fc-His tag was a gift from Alexander McLellan (Addgene plasmid # 183253 ; http://n2t.net/addgene:183253 ; RRID:Addgene_183253)