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Addgene

pcDNA3.1(-) EC Her2-Fc
(Plasmid #183250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Her2 antigen Fc
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-) EC Her2-Fc was a gift from Alexander McLellan (Addgene plasmid # 183250 ; http://n2t.net/addgene:183250 ; RRID:Addgene_183250)

  • For your References section:

    Promoter choice: Who should drive the CAR in T cells?. Rad S M AH, Poudel A, Tan GMY, McLellan AD. PLoS One. 2020 Jul 24;15(7):e0232915. doi: 10.1371/journal.pone.0232915. eCollection 2020. 10.1371/journal.pone.0232915 PubMed 32706785
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