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PurposeExpression vector for PUF9R-mRuby2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmax
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Backbone manufacturerLonza
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePUF9R-mRuby2
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SpeciesSynthetic
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC11003-pmax-PUF9R_mRuby2 was a gift from Albert Cheng (Addgene plasmid # 183210) -
For your References section:
CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locus. Clow PA, Du M, Jillette N, Taghbalout A, Zhu JJ, Cheng AW. Nat Commun. 2022 Apr 6;13(1):1871. doi: 10.1038/s41467-022-29343-z. 10.1038/s41467-022-29343-z PubMed 35387989