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PurposeLentiviral vector for constitutive dCas9 expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonelenti_Cas9
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9
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SpeciesSynthetic
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Insert Size (bp)-3
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MutationH840A; D10A for nuclease deficiency
Resource Information
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A portion of this plasmid was derived from a plasmid made bymodified from pX330
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC11001-lenti_dCas9 was a gift from Albert Cheng (Addgene plasmid # 183208) -
For your References section:
CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locus. Clow PA, Du M, Jillette N, Taghbalout A, Zhu JJ, Cheng AW. Nat Commun. 2022 Apr 6;13(1):1871. doi: 10.1038/s41467-022-29343-z. 10.1038/s41467-022-29343-z PubMed 35387989