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Addgene

pAC11001-lenti_dCas9
(Plasmid #183208)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183208 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    lenti_Cas9
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Synthetic
  • Insert Size (bp)
    -3
  • Mutation
    H840A; D10A for nuclease deficiency

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    modified from pX330

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC11001-lenti_dCas9 was a gift from Albert Cheng (Addgene plasmid # 183208)
  • For your References section:

    CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locus. Clow PA, Du M, Jillette N, Taghbalout A, Zhu JJ, Cheng AW. Nat Commun. 2022 Apr 6;13(1):1871. doi: 10.1038/s41467-022-29343-z. 10.1038/s41467-022-29343-z PubMed 35387989