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Addgene

pDONR221 Cas9-HMGB1
(Plasmid #183198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221 (2184742_AE175)
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Vector type
    CRISPR ; Gateway entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-HMGB1
  • Species
    Synthetic
  • Mutation
    n/a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221 Cas9-HMGB1 was a gift from Emma Haapaniemi (Addgene plasmid # 183198 ; http://n2t.net/addgene:183198 ; RRID:Addgene_183198)

  • For your References section:

    Rapid genome editing by CRISPR-Cas9-POLD3 fusion. Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, Haapaniemi E. Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415. 10.7554/eLife.75415 PubMed 34898428
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