NSSCP
(Plasmid
#183190)
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PurposemRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB1C3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namemRFP-PopZ
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Insert Size (bp)1247
Gene/Insert 2
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Gene/Insert nameDivIVA-GFP(tevR-50)
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Insert Size (bp)1287
Gene/Insert 3
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Gene/Insert nameSpmX-TEV(C)
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Insert Size (bp)867
Gene/Insert 4
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Gene/Insert nameTEV(N)-SpmX
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Insert Size (bp)831
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NSSCP was a gift from Hsiao-Chun Huang (Addgene plasmid # 183190 ; http://n2t.net/addgene:183190 ; RRID:Addgene_183190) -
For your References section:
Localized Proteolysis for the Construction of Intracellular Asymmetry in Escherichia coli. Hong JC, Fan HC, Yang PJ, Lin DW, Wu HC, Huang HC. ACS Synth Biol. 2021 Aug 20;10(8):1830-1836. doi: 10.1021/acssynbio.1c00200. Epub 2021 Aug 10. 10.1021/acssynbio.1c00200 PubMed 34374512