pNSSCPG
(Plasmid #183187)

• Purpose: PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
• Depositing Lab: Hsiao-Chun Huang
• Publication: Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 10.1038/s41467-021-21135-1.

Backbone

• Vector backbone: pSB1C3
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: superfolder GFP
• Insert Size (bp): 713

Gene/Insert 2

• Gene/Insert name: SpmX450 and T7 181+ fusion protein
• Insert Size (bp): 2598

Gene/Insert 3

• Gene/Insert name: T7 RNAP 1-179 variant and SpmX450 fusion protein
• Insert Size (bp): 1017

Gene/Insert 4

• Gene/Insert name: mRFP1 and popZ fusion protein
• Insert Size (bp): 1230

Resource Information

• Supplemental Documents:
  • pNSSCPG.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry