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Addgene

pcDNA3.1+_hsTRPA1_myc_dTom
(Plasmid #183179)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183179 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1+/C-(K)DYK
  • Backbone size w/o insert (bp) 3361
  • Total vector size (bp) 10407
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    hsTRPA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3357
  • GenBank ID
    NM_007332.3
  • Entrez Gene
    TRPA1 (a.k.a. ANKTM1, FEPS, FEPS1, p120)

Gene/Insert 2

  • Gene/Insert name
    dTom
  • Species
    Discosoma coral
  • Insert Size (bp)
    1425
  • GenBank ID
    LC311026.1

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1+_hsTRPA1_myc_dTom was a gift from Sreekanth Chalasani (Addgene plasmid # 183179 ; http://n2t.net/addgene:183179 ; RRID:Addgene_183179)
  • For your References section:

    Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels. Duque M, Lee-Kubli CA, Tufail Y, Magaram U, Patel J, Chakraborty A, Mendoza Lopez J, Edsinger E, Vasan A, Shiao R, Weiss C, Friend J, Chalasani SH. Nat Commun. 2022 Feb 9;13(1):600. doi: 10.1038/s41467-022-28205-y. 10.1038/s41467-022-28205-y PubMed 35140203