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Purposemyc tagged hsTRPA1 used for cell transfection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1+/C-(K)DYK
- Backbone size w/o insert (bp) 3361
- Total vector size (bp) 10407
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namehsTRPA1
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SpeciesH. sapiens (human)
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Insert Size (bp)3357
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GenBank IDNM_007332.3
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Entrez GeneTRPA1 (a.k.a. ANKTM1, FEPS, FEPS1, p120)
Gene/Insert 2
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Gene/Insert namedTom
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SpeciesDiscosoma coral
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Insert Size (bp)1425
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GenBank IDLC311026.1
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1+_hsTRPA1_myc_dTom was a gift from Sreekanth Chalasani (Addgene plasmid # 183179 ; http://n2t.net/addgene:183179 ; RRID:Addgene_183179) -
For your References section:
Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels. Duque M, Lee-Kubli CA, Tufail Y, Magaram U, Patel J, Chakraborty A, Mendoza Lopez J, Edsinger E, Vasan A, Shiao R, Weiss C, Friend J, Chalasani SH. Nat Commun. 2022 Feb 9;13(1):600. doi: 10.1038/s41467-022-28205-y. 10.1038/s41467-022-28205-y PubMed 35140203