pJS647
(Plasmid
#183176)
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PurposePYR1 mutagenesis plasmid compatible with pJS646 Y2H vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYTK084
- Backbone size w/o insert (bp) 1774
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePYR1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)408
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Entrez GenePYR1 (a.k.a. AT4G17870, PYRABACTIN RESISTANCE 1, RCAR11, T6K21.50, T6K21_50, regulatory component of ABA receptor 11)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJS647 was a gift from Timothy Whitehead (Addgene plasmid # 183176 ; http://n2t.net/addgene:183176 ; RRID:Addgene_183176) -
For your References section:
Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds. Beltran J, Steiner PJ, Bedewitz M, Wei S, Peterson FC, Li Z, Hughes BE, Hartley Z, Robertson NR, Medina-Cucurella AV, Baumer ZT, Leonard AC, Park SY, Volkman BF, Nusinow DA, Zhong W, Wheeldon I, Cutler SR, Whitehead TA. Nat Biotechnol. 2022 Jun 20. pii: 10.1038/s41587-022-01364-5. doi: 10.1038/s41587-022-01364-5. 10.1038/s41587-022-01364-5 PubMed 35726092
