pUC19/mChe-PM
(Plasmid
#183168)
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PurposeConstruct for transient expression of plasma membrane-targeted mCherry in plants.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-PM
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Alt nameplasma membrane-targeted mCherry
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SpeciesSynthetic
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Tag
/ Fusion Protein
- OsRac3, O. sativa rac-like GTP-binding protein 3 (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a G17V mutation in OsRac3 compared to the NCBI reference sequence XP_015625155.1. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19/mChe-PM was a gift from Yao-Guang Liu & Qinlong Zhu (Addgene plasmid # 183168 ; http://n2t.net/addgene:183168 ; RRID:Addgene_183168) -
For your References section:
All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for Protein Localization and BiFC Analyses in Plants. Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu YG, Zhu Q. Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790. 10.1111/pbi.13790 PubMed 35179286