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Addgene

pUC19/ER-mChe
(Plasmid #183163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER-mCherry
  • Alt name
    endoplasmic reticulum-targeted mCherry
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • 25 aa ER signal of Glycine soja kunitz-type trypsin inhibitor (KTI1) (N terminal on insert)
    • ER retention signal peptide HDEL (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19/ER-mChe was a gift from Yao-Guang Liu & Qinlong Zhu (Addgene plasmid # 183163 ; http://n2t.net/addgene:183163 ; RRID:Addgene_183163)

  • For your References section:

    All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for Protein Localization and BiFC Analyses in Plants. Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu YG, Zhu Q. Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790. 10.1111/pbi.13790 PubMed 35179286
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