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p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
(Plasmid #183150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HO-Poly-KanMX4-HO
  • Backbone manufacturer
    Voth et al., 2001
  • Vector type
    For yeast genomic integration
  • Selectable markers
    Nourseothricin (cloNAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Lex-ER-B112
  • Species
    Synthetic

Gene/Insert 2

  • Gene/Insert name
    Zif268 DBD - hPRLBD - MSN2AD
  • Alt name
    ZPM
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digest with PvuII for obtaining linear fragment for genomic integration to yeast into site 20 (Flagfeldt et al., 2009) via homologous recombination.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM was a gift from Assaf Mosquna (Addgene plasmid # 183150 ; http://n2t.net/addgene:183150 ; RRID:Addgene_183150)

  • For your References section:

    A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein-Protein Interactions. Feuer E, Zimran G, Shpilman M, Mosquna A. ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. 10.1021/acssynbio.2c00192 PubMed 35895499
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