pBD-pZ-FLAG
(Plasmid
#183148)
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PurposeYeast-two-hybrid BD vector with pZ promoter (activated by ZPM)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBD-GAL4
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Backbone manufacturerClontech
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Vector typeYeast Expression ; Yeast two-hybrid
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGAL4 BD
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBD-pZ-FLAG was a gift from Assaf Mosquna (Addgene plasmid # 183148 ; http://n2t.net/addgene:183148 ; RRID:Addgene_183148) -
For your References section:
A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein-Protein Interactions. Feuer E, Zimran G, Shpilman M, Mosquna A. ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. 10.1021/acssynbio.2c00192 PubMed 35895499
Map uploaded by the depositor.
