pHO-UAS-mScarlet-I
(Plasmid
#183147)
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PurposeYeat two-hybrid fluorecent reporter for genomic integration
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneHO-Poly-KanMX4-HO
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Backbone manufacturerVoth et al., 2001
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemScarlet-I
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digest with NotI for obtaining linear fragment for genomic integration to yeast via homologous recombination.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHO-UAS-mScarlet-I was a gift from Assaf Mosquna (Addgene plasmid # 183147 ; http://n2t.net/addgene:183147 ; RRID:Addgene_183147) -
For your References section:
A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein-Protein Interactions. Feuer E, Zimran G, Shpilman M, Mosquna A. ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. 10.1021/acssynbio.2c00192 PubMed 35895499
Map uploaded by the depositor.
