promoter-less mRFP1E
(Plasmid
#183144)
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PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBioBrick pSB1C3 vector
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Backbone manufactureriGEM Registry
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Modifications to backboneBioBrick sites removed
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBS, mRFP1E
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SpeciesSynthetic
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Insert Size (bp)767
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MutationBioBrick sites removed
Resource Information
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A portion of this plasmid was derived from a plasmid made byOriginally from iGEM Registry BBa_I13502
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For information: http://parts.igem.org/Part:BBa_I13502
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
promoter-less mRFP1E was a gift from Anthony Forster (Addgene plasmid # 183144 ; http://n2t.net/addgene:183144 ; RRID:Addgene_183144) -
For your References section:
Overcoming chromoprotein limitations by engineering a red fluorescent protein. Bao L, Menon PNK, Liljeruhm J, Forster AC. Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936. 10.1016/j.ab.2020.113936 PubMed 32891596
