pBTK1024 (pDS-Def1)
(Plasmid
#183125)
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PurposeExpresses double-stranded RNA targeting the Def1 gene from Apis mellifera
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRSF1010
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFragment of Def1 gene
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SpeciesApis mellifera
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Insert Size (bp)184
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GenBank IDNM_001011616.2
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTK1024 (pDS-Def1) was a gift from Jeffrey Barrick (Addgene plasmid # 183125 ; http://n2t.net/addgene:183125 ; RRID:Addgene_183125) -
For your References section:
Honey bee functional genomics using symbiont-mediated RNAi. Lariviere PJ, Leonard SP, Horak RD, Powell JE, Barrick JE. Nat Protoc. 2023 Mar;18(3):902-928. doi: 10.1038/s41596-022-00778-4. Epub 2022 Dec 2. 10.1038/s41596-022-00778-4 PubMed 36460809