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Addgene

BA-Wnt10b
(Plasmid #1831)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnt10b
  • Alt name
    Wnt-10b
  • Alt name
    wingless related MMTV integration site 10b
  • Alt name
    beta-actin promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3400
  • Entrez Gene
    Wnt10b (a.k.a. Wnt1, Wnt12)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See scanned map.
(Leder #C-893)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BA-Wnt10b was a gift from Philip Leder (Addgene plasmid # 1831 ; http://n2t.net/addgene:1831 ; RRID:Addgene_1831)

  • For your References section:

    Wnt-10b directs hypermorphic development and transformation in mammary glands of male and female mice. Lane TF, Leder P. Oncogene 1997 Oct;15(18):2133-44. 10.1038/sj.onc.1201593 PubMed 9393971
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