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Addgene

pBY011-AMN1ko
(Plasmid #183099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBY011
  • Backbone manufacturer
    Harvard Medical School
  • Backbone size w/o insert (bp) 3607
  • Total vector size (bp) 5972
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm)
  • Alt name
    AMN1 (YBR158W)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2365
  • Mutation
    only includes external sequences (504 bp each) as homology arms, separated by KanMX
  • GenBank ID
    EEU05423 EEU05423.1
  • Entrez Gene
    AMN1 (a.k.a. YBR158W, CST13, ICS4)
  • Promoter URA3, TEF1
  • Tag / Fusion Protein
    • KanMX

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAACTAACAAAAACGTTCAAAAAGTTT
  • 3′ sequencing primer AACACTATGAGTGTATCTTTTGGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBY011-AMN1ko was a gift from Gabor Balazsi (Addgene plasmid # 183099 ; http://n2t.net/addgene:183099 ; RRID:Addgene_183099)

  • For your References section:

    Drug-dependent growth curve reshaping reveals mechanisms of antifungal resistance in Saccharomyces cerevisiae. Guinn L, Lo E, Balazsi G. Commun Biol. 2022 Mar 31;5(1):292. doi: 10.1038/s42003-022-03228-9. 10.1038/s42003-022-03228-9 PubMed 35361876
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