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Addgene

pMVS-V1
(Plasmid #183094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTL80150
  • Backbone manufacturer
    https://plasmidvectors.com (Nigel Minton lab)
  • Backbone size w/o insert (bp) 2360
  • Total vector size (bp) 8035
  • Vector type
    bacterial propagation and conjugative

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pME2001
  • Alt name
    pMTBMA4
  • Species
    Methanothermobacter marburgensis
  • Insert Size (bp)
    4434
  • GenBank ID
    CP001711.1

Gene/Insert 2

  • Gene/Insert name
    thermostable neomycin phosphotransferase gene
  • Alt name
    NeoR
  • Insert Size (bp)
    1241
  • GenBank ID

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMTL83151: Heap JT, Pennington OJ, Cartman ST, Minton NP. 2009. A modular system
for Clostridium shuttle plasmids. J Microbiol Methods 78:79–85. https://
doi.org/10.1016/j.mimet.2009.05.004.

thermostable NeoR: pMU131 (Addgene #66811)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMVS-V1 was a gift from Lars Angenent & Bastian Molitor (Addgene plasmid # 183094 ; http://n2t.net/addgene:183094 ; RRID:Addgene_183094)

  • For your References section:

    A Shuttle-Vector System Allows Heterologous Gene Expression in the Thermophilic Methanogen Methanothermobacter thermautotrophicus DeltaH. Fink C, Beblawy S, Enkerlin AM, Muhling L, Angenent LT, Molitor B. mBio. 2021 Dec 21;12(6):e0276621. doi: 10.1128/mBio.02766-21. Epub 2021 Nov 23. 10.1128/mBio.02766-21 PubMed 34809461
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