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Addgene

pB025
(Plasmid #183092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB006
  • Backbone size w/o insert (bp) 5025
  • Total vector size (bp) 12371
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Erythromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E.coli S17-1
  • Growth instructions
    LB
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FnCas12a, gRNA, Promoter, HAB, TetR,
  • gRNA/shRNA sequence
    TACTATATTTCCCTTCCCTTTCAGA
  • Promoter P1TDPGH023

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cggctgacatgggaattcccctccaccgcggtggcttaagacccactttc
  • 3′ sequencing primer gaattttgatggattcatacaagcggtcggccttaaagcctccagttgttcggaatt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB025 was a gift from Lei Dai (Addgene plasmid # 183092 ; http://n2t.net/addgene:183092 ; RRID:Addgene_183092)

  • For your References section:

    CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species. Zheng L, Tan Y, Hu Y, Shen J, Qu Z, Chen X, Ho CL, Leung EL, Zhao W, Dai L. ACS Synth Biol. 2022 Jan 21;11(1):464-472. doi: 10.1021/acssynbio.1c00543. Epub 2022 Jan 6. 10.1021/acssynbio.1c00543 PubMed 34990118
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