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Addgene

pYTK001_E2At(2nd)
(Plasmid #183085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Backbone manufacturer
    John Dueber
  • Backbone size w/o insert (bp) 1666
  • Total vector size (bp) 1726
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E2A
  • Alt name
    ERBV-1
  • Species
    Synthetic; Equine rhinitis B virus
  • Insert Size (bp)
    60

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CCTTTTTACGGTTCCTGGCC
  • 3′ sequencing primer CTTGGACTCCTGTTGATAGATCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYTK001_E2At(2nd) was a gift from Zhen Wang (Addgene plasmid # 183085 ; http://n2t.net/addgene:183085 ; RRID:Addgene_183085)

  • For your References section:

    A Well-Characterized Polycistronic-Like Gene Expression System in Yeast. Mukherjee M, Wang ZQ. Biotechnol Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247. 10.1002/bit.28247 PubMed 36168285
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