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pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb
(Plasmid #183078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Received from R. Platt from F. Zhang lab, Broad Institute
  • Backbone size w/o insert (bp) 4153
  • Total vector size (bp) 5990
  • Modifications to backbone
    Replaced all sequence between AAV ITRs with insert acceptance site and PGK-Puro-PolyA.
  • Vector type
    AAV ; Homologous Recombination Donor Template
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms
  • Alt name
    BRAF Exon 15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1837
  • Mutation
    V600E (T>A), S607S (TCC>AGT)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Promoter None for primary insert. PGK for puromycin resistance gene.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer NGS
  • 3′ sequencing primer NGS
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb was a gift from Aviv Regev (Addgene plasmid # 183078 ; http://n2t.net/addgene:183078 ; RRID:Addgene_183078)

  • For your References section:

    Stepwise-edited, human melanoma models reveal mutations' effect on tumor and microenvironment. Hodis E, Torlai Triglia E, Kwon JYH, Biancalani T, Zakka LR, Parkar S, Hutter JC, Buffoni L, Delorey TM, Phillips D, Dionne D, Nguyen LT, Schapiro D, Maliga Z, Jacobson CA, Hendel A, Rozenblatt-Rosen O, Mihm MC Jr, Garraway LA, Regev A. Science. 2022 Apr 29;376(6592):eabi8175. doi: 10.1126/science.abi8175. Epub 2022 Apr 29. 10.1126/science.abi8175 PubMed 35482859
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