pCJH059_proD_Cas12c_DualRNA-GFP_CAM
(Plasmid
#183076)
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PurposePlasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A ori
- Total vector size (bp) 3556
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCas12c tracrRNA and pre-crRNA
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFunctionally diverse type V CRISPR-Cas systems. Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng DR, Scott DA. Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. Epub 2018 Dec 6. 10.1126/science.aav7271 PubMed 30523077
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCJH059_proD_Cas12c_DualRNA-GFP_CAM was a gift from Jennifer Doudna (Addgene plasmid # 183076 ; http://n2t.net/addgene:183076 ; RRID:Addgene_183076) -
For your References section:
A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression. Huang CJ, Adler BA, Doudna JA. Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. 10.1016/j.molcel.2022.04.020 PubMed 35659325