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pCJH027_SC101_pLAC_LbCas12a_Kan
(Plasmid #183074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101 ori
  • Total vector size (bp) 8563
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LbCas12a
  • Species
    Lachnospiraceae bacterium
  • Insert Size (bp)
    3687
  • Mutation
    Wild-type

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA. Science. 2018 Apr 27;360(6387):436-439. doi: 10.1126/science.aar6245. Epub 2018 Feb 15. 10.1126/science.aar6245 PubMed 29449511

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCJH027_SC101_pLAC_LbCas12a_Kan was a gift from Jennifer Doudna (Addgene plasmid # 183074 ; http://n2t.net/addgene:183074 ; RRID:Addgene_183074)

  • For your References section:

    A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression. Huang CJ, Adler BA, Doudna JA. Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. 10.1016/j.molcel.2022.04.020 PubMed 35659325
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