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pCJH021_SC101_pLAC_Cas12c2_Kan
(Plasmid #183072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101 ori
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas12c2
  • Alt name
    UnCas12c2
  • Species
    Synthetic
  • Insert Size (bp)
    3657

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Functionally diverse type V CRISPR-Cas systems. Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng DR, Scott DA. Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. Epub 2018 Dec 6. 10.1126/science.aav7271 PubMed 30523077

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Cas12c2 sequence was from pET28a-mH6-Cas12c2 (Addgene plasmid # 120873 ; http://n2t.net/addgene:120873 ; RRID:Addgene_120873)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCJH021_SC101_pLAC_Cas12c2_Kan was a gift from Jennifer Doudna (Addgene plasmid # 183072 ; http://n2t.net/addgene:183072 ; RRID:Addgene_183072)

  • For your References section:

    A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression. Huang CJ, Adler BA, Doudna JA. Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. 10.1016/j.molcel.2022.04.020 PubMed 35659325
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