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Addgene

pBRC1
(Plasmid #183064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1MCS2
  • Backbone size w/o insert (bp) -1
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eSpCas9ppD10A

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBRC1 was a gift from Xuehong Zhang (Addgene plasmid # 183064 ; http://n2t.net/addgene:183064 ; RRID:Addgene_183064)

  • For your References section:

    Developing a CRISPR-assisted base-editing system for genome engineering of Pseudomonas chlororaphis. Yue SJ, Huang P, Li S, Cai YY, Wang W, Zhang XH, Nikel PI, Hu HB. Microb Biotechnol. 2022 Sep;15(9):2324-2336. doi: 10.1111/1751-7915.14075. Epub 2022 May 16. 10.1111/1751-7915.14075 PubMed 35575623
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