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pMM2_10
(Plasmid #183059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MoClo-YTK
  • Total vector size (bp) 9866
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Promoter Synthetic promoter
  • Tag / Fusion Protein
    • mCherry

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    HAA1-mTurquoise2
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Promoter HAA1
  • Tag / Fusion Protein
    • mTurquoise2

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid parts were synthesized or taken from the Yeast MoClo kit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM2_10 was a gift from Yvonne Nygård (Addgene plasmid # 183059 ; http://n2t.net/addgene:183059 ; RRID:Addgene_183059)

  • For your References section:

    Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae. Mormino M, Siewers V, Nygard Y. FEMS Yeast Res. 2021 Sep 11;21(6). pii: 6363685. doi: 10.1093/femsyr/foab049. 10.1093/femsyr/foab049 PubMed 34477863
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