Skip to main content
Addgene

pMM2_4
(Plasmid #183057)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MoClo-YTK
  • Total vector size (bp) 9868
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mRuby2
  • Species
    Synthetic
  • Promoter YGP1
  • Tag / Fusion Protein
    • mRuby2

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    HAA1-mTurquoise2
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Promoter HAA1
  • Tag / Fusion Protein
    • mTurquoise2

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid parts were synthesized or taken from the Yeast MoClo kit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM2_4 was a gift from Yvonne Nygård (Addgene plasmid # 183057 ; http://n2t.net/addgene:183057 ; RRID:Addgene_183057)

  • For your References section:

    Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae. Mormino M, Siewers V, Nygard Y. FEMS Yeast Res. 2021 Sep 11;21(6). pii: 6363685. doi: 10.1093/femsyr/foab049. 10.1093/femsyr/foab049 PubMed 34477863
Related items:
Commonly requested with: