pLenti_CMV:Flag-CeNL_SV40:PuroR
(Plasmid
#183039)
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PurposeExpresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCeNL Nanolantern
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SpeciesSynthetic
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Insert Size (bp)1197
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_CMV:Flag-CeNL_SV40:PuroR was a gift from Antonio Amelio (Addgene plasmid # 183039 ; http://n2t.net/addgene:183039 ; RRID:Addgene_183039) -
For your References section:
Multiplexed bioluminescence microscopy via phasor analysis. Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA. Nat Methods. 2022 Jul;19(7):893-898. doi: 10.1038/s41592-022-01529-9. Epub 2022 Jun 23. 10.1038/s41592-022-01529-9 PubMed 35739310