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Addgene

pLenti_CMV:Flag-GpNLuc_SV40:PuroR
(Plasmid #183037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GpNLuc LumiFluor
  • Species
    Synthetic
  • Insert Size (bp)
    1245
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_CMV:Flag-GpNLuc_SV40:PuroR was a gift from Antonio Amelio (Addgene plasmid # 183037 ; http://n2t.net/addgene:183037 ; RRID:Addgene_183037)

  • For your References section:

    Multiplexed bioluminescence microscopy via phasor analysis. Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA. Nat Methods. 2022 Jul;19(7):893-898. doi: 10.1038/s41592-022-01529-9. Epub 2022 Jun 23. 10.1038/s41592-022-01529-9 PubMed 35739310
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