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Addgene

pET-11-LECT2-I40
(Plasmid #183032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11
  • Backbone manufacturer
    Novagen/EMD
  • Backbone size w/o insert (bp) 5677
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LECT2
  • Alt name
    Leukocyte chemotactic factor 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    405
  • Mutation
    I40 variant
  • Entrez Gene
    LECT2 (a.k.a. chm-II, chm2)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7_term
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned from a synthetic fragment (Twist Biosciences)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.03.01.482540 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-11-LECT2-I40 was a gift from Gareth Morgan (Addgene plasmid # 183032 ; http://n2t.net/addgene:183032 ; RRID:Addgene_183032)

  • For your References section:

    An amyloidosis-associated polymorphism does not alter LECT2 stability in vitro. Belonogov L, Taylor PE, Wong S, Morgan GJ. bioRxiv 2022.03.01.482540 10.1101/2022.03.01.482540
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