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pCapVQ329R
(Plasmid #183029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD28
  • Backbone manufacturer
    Beckwith Lab
  • Backbone size w/o insert (bp) 5801
  • Total vector size (bp) 6885
  • Vector type
    Bacterial Expression
  • Selectable markers
    chloramphenicol

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    upon induction of CapVQ329R in Escherichia coli MG1655 and other strains, extensive filamentation is induced
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    patatin-like phospholipase variant CapVQ329R
  • Alt name
    locus tag of wild type CapV: BHF03_RS01975
  • Species
    Escherichia coli ECOR31
  • Insert Size (bp)
    1102
  • Mutation
    glutamine 329 changed to arginine
  • GenBank ID
    GCA_001865905.1
  • Promoter pBAD promoter
  • Tag / Fusion Protein
    • No

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer VC0178-81-SacI-fw GGCGAGCTCGCTATATTCTCTGGTTATGGGGTTTTCAATGTCTG
  • 3′ sequencing primer VC0178-XbaI-rv GCTCTAGATCAGAGTTTCTCCTGCGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCapVQ329R was a gift from Ute Romling (Addgene plasmid # 183029 ; http://n2t.net/addgene:183029 ; RRID:Addgene_183029)

  • For your References section:

    Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution. Li F, Cao L, Bahre H, Kim SK, Schroeder K, Jonas K, Koonce K, Mekonnen SA, Mohanty S, Bai F, Brauner A, Lee VT, Rohde M, Romling U. NPJ Biofilms Microbiomes. 2022 May 11;8(1):39. doi: 10.1038/s41522-022-00294-z. 10.1038/s41522-022-00294-z PubMed 35546554
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