pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA
(Plasmid
#183025)
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PurposeB6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Backbone manufacturerHyung-song Nam, Capecchi laboratory
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Vector typeMammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nametdTomato
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCAG promoter is from pCAGGS obtained from BCCM/LMBP. FNF is from plasmid #22687. bGH pA is from Neal Copeland. SV40 pA is from Clontech. tdTomato is from Roger Tsien. WPRE is from Miguel Ramalho-Santos.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA was a gift from Mario Capecchi (Addgene plasmid # 183025 ; http://n2t.net/addgene:183025 ; RRID:Addgene_183025)