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Addgene

pFA6a-FKBP-GFP-HIS3MX6
(Plasmid #183023)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183023 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFA6a-GFP(S65T)-HIS3MX6
  • Backbone size w/o insert (bp) 4698
  • Total vector size (bp) 5049
  • Vector type
    Yeast Genomic Targeting
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FKBP
  • Alt name
    FKBP12
  • Alt name
    FKBP (F36V)
  • Alt name
    DmrB
  • Species
    Synthetic
  • Insert Size (bp)
    351

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-FKBP-GFP-HIS3MX6 was a gift from Santiago Di Pietro (Addgene plasmid # 183023 ; http://n2t.net/addgene:183023 ; RRID:Addgene_183023)
  • For your References section:

    Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast. Lamb AK, Di Pietro SM. STAR Protoc. 2022 Apr 14;3(2):101323. doi: 10.1016/j.xpro.2022.101323. eCollection 2022 Jun 17. 10.1016/j.xpro.2022.101323 PubMed 35496798