pFA6a-FKBP-GFP-HIS3MX6
(Plasmid
#183023)
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PurposePCR-based tagging of yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a-GFP(S65T)-HIS3MX6
- Backbone size w/o insert (bp) 4698
- Total vector size (bp) 5049
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Vector typeYeast Genomic Targeting
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP
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Alt nameFKBP12
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Alt nameFKBP (F36V)
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Alt nameDmrB
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SpeciesSynthetic
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Insert Size (bp)351
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-FKBP-GFP-HIS3MX6 was a gift from Santiago Di Pietro (Addgene plasmid # 183023 ; http://n2t.net/addgene:183023 ; RRID:Addgene_183023) -
For your References section:
Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast. Lamb AK, Di Pietro SM. STAR Protoc. 2022 Apr 14;3(2):101323. doi: 10.1016/j.xpro.2022.101323. eCollection 2022 Jun 17. 10.1016/j.xpro.2022.101323 PubMed 35496798