Anti-Kv1.3 K+ channel [L23/27R]
(Antibody
#183000)
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PurposeAnti-Kv1.3 K+ channel (Rat) recombinant mouse monoclonal antibody
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Depositing Lab
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Recommended Applications
Image 1 (of 2): Western Blot
Crude rat membranes immunoblotted against Anti-Kv1.3 K+ channel hybridoma-derived antibod…
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Credit: James Trimmer, UC Davis
Image 2 (of 2): Immunohistochemistry
Free-floating rat brain sections were fixed and stained with purified recombinant Anti-Kv…
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Credit: James Trimmer, UC Davis
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Recombinant Antibody | 183000-rAb | 100 µg of purified recombinant antibody | $250 * | ||
| Recombinant Antibody trial size | 183000-rAb.T | 20 µg of purified recombinant antibody | $85 * | ||
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Features
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Source SpeciesM. musculus (mouse)
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IsotypeIgG2a
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Light Chain Typekappa
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Amino Acid Sequences
Sequences (4) — Accept Affinity Reagent Sequence Policy
Production & Usage
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Production PlasmidAnti-Kv1.3 K+ channel [L23/27R]
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Antigen PlasmidKv1.3/RBG4
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PurificationPurified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
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Storage BufferPBS + 1 mM sodium azide
Delivery
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Concentration0.9–1.1 mg/mL
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StorageUpon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
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ShipmentBlue ice or room temp
Resource Information
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Persistent Resource IdentifierRRID:AB_2909549
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Zeocin® is an InvivoGen trademark.
Trademarks:
Target Antigen
| Protein | Potassium voltage-gated channel subfamily A member 3 |
|---|---|
| Antigen Description | Amino acids 485–506 (cytoplasmic C-terminus) of rat Kv1.3 |
| Antigen Amino Acid Sequence |
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| Species | R. norvegicus (rat) |
| Additional Species Reactivity | H. sapiens (human), M. musculus (mouse) |
| Gene | Kcna3 |
| Alternative Names |
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| External References |
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Applications (5)
Immunohistochemistry
Western Blot
Knockout validation
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-Kv1.3 K+ channel [L23/27R] - from James Trimmer (Addgene antibody # 183000 ; http://n2t.net/addgene:183000 ; RRID:AB_2909549) -
For your References section:
High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758930