Full-length P229S talin1
(Plasmid
#182993)
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Purposeexpress FL variant P229S talin1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Total vector size (bp) 12357
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTalin1 P229S
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SpeciesM. musculus (mouse)
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MutationP229S
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Entrez GeneTln1 (a.k.a. Tln)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Full-length P229S talin1 was a gift from Vesa Hytönen (Addgene plasmid # 182993 ; http://n2t.net/addgene:182993 ; RRID:Addgene_182993) -
For your References section:
Talin variant P229S compromises integrin activation and associates with multifaceted clinical symptoms. Azizi L, Varela L, Turkki P, Mykuliak VV, Korpela S, Ihalainen TO, Church J, Hytonen VP, Goult BT. Hum Mol Genet. 2022 Jul 21. pii: 6647920. doi: 10.1093/hmg/ddac163. 10.1093/hmg/ddac163 PubMed 35861643