pKT2/CAGXSP/PalmReNL
(Plasmid
#182970)
-
PurposeStably express PalmReNL in mammalian cells via the sleeping beauty transposon system.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 182970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepKT2/CAGXSP
-
Backbone manufacturerMichael H. Bachmann
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePalmReNL
-
SpeciesSynthetic
-
MutationN/A
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKT2/CAGXSP/PalmReNL was a gift from Masamitsu Kanada (Addgene plasmid # 182970 ; http://n2t.net/addgene:182970 ; RRID:Addgene_182970) -
For your References section:
In Vitro and In Vivo Analysis of Extracellular Vesicle-Mediated Metastasis Using a Bright, Red-Shifted Bioluminescent Reporter Protein. Perez GI, Broadbent D, Zarea AA, Dolgikh B, Bernard MP, Withrow A, McGill A, Toomajian V, Thampy LK, Harkema J, Walker JR, Kirkland TA, Bachmann MH, Schmidt J, Kanada M. Adv Genet (Hoboken). 2022 Jan 19;3(1):2100055. doi: 10.1002/ggn2.202100055. eCollection 2022 Mar. 10.1002/ggn2.202100055 PubMed 36619349